Biomimicry of bone formation process achieved in a laboratory

Bone consists of fibers of collagen in which calcium phosphate is deposited in the form of nanocrystals. The team of dr. Nico Sommerdijk (Department of Chemical Engineering and Chemistry) consisting of researchers from Eindhoven University of Technology (TU/e) and the University of Illinois was able to mimic the growth of calcium phosphate inside the collagen in the laboratory, just as it happens in the human body.

For a long time it was thought that collagen was only a template for the deposition of calcium phosphate, and that bone formation was controlled by specialized biomolecules. However, the images taken by the Eindhoven researchers show that the collagen fibers themselves control the mineral formation process and thereby direct bone formation. The biomolecules have proved to have a different role in the mineralization process – they keep the calcium phosphate in solution until mineral growth starts.

The team visualized this process using a unique electron microscope, the cryoTitan. This microscope allowed the researchers to investigate samples that were very rapidly frozen, so that the process could be arrested and viewed in steps. The cryoTitan has an extremely high resolution, and can even distinguish single atoms.

An Italian research institute (ISTEC) is already developing new bone implants based on the knowledge gained by Sommerdijk and his post-doc Fabio Nudelman. Sommerdijk’s group does not intend to take this step towards production: “We have taken a big step forwards in the area of bone formation, but our interest is in understanding, not production.”

The newly gained knowledge about bone formation has opened the door to a new research area for Sommerdijk’s group. He is confident that the same principles can be used to make various kinds of nanomaterials. Sommerdijk and Nudelman are starting with magnetite, a magnetic material that can be used as biomarker or for data storage. But their ambitions go even further.

“I am seriously convinced that we can make all kinds of materials using these principles”, said Sommerdijk. He is very enthusiastic about the new research direction. “The biomimetic formation of magnetic materials is a new area that is still completely unexplored.”

The paper “The role of collagen in bone apatite formation in the presence of hydroxyapatite nucleation inhibitors” is describing the results is available on the Nature Materials website from 24 October, and will be published in the December edition of the journal.